Molecular cloning and characterization of three CoIDA genes in Camellia oleifera
نویسندگان
چکیده
The INFLORESCENCE DEFICIENT IN ABSCISSION (IDA) controls floral organ abscission in plants. IDA belongs to IDA-LIKE (IDL) gene family that is involved regulation of Arabidopsis development. Herein, we identified three genes, CoIDA1, CoIDA2 and CoIDA3 Camellia oleifera (Camellia Abel. cv. Huashuo) suggested their involvement the fruits abscission. full-length cDNA sequences were 207 bp, 276 bp 273 encoding proteins 68, 91 90 amino acids, respectively. These CoIDA genes single exon (SEGs) with a conserved extended PIP motif (EPIP) at C-terminal has been implicated play an important role governing protein function for enhanced flower abortion rate. highest expression CoIDA1 was young peduncles lowest fruits. However, expressions both roots, significantly increased zones (AZs) abnormal (AF) ethephon treated (ETH-F) respect normal (NF), which suggest are related C. oleifera. This study provided preliminary understanding about could lead detailed functional analysis utilization improving yield potential.
منابع مشابه
Transcriptome analysis of the tea oil camellia (Camellia oleifera) reveals candidate drought stress genes
BACKGROUND The tea-oil camellia (Camellia oleifera) is the most important oil plant in southern China, and has a strong resistance to drought and barren soil. Understanding the molecular mechanisms of drought tolerance would greatly promote its cultivation and molecular breeding. RESULTS In total, we obtained 76,585 unigenes with an average length of 810 bp and an N50 of 1,092 bp. We mapped a...
متن کاملMolecular cloning, characterization, and expression of pannexin genes in chicken.
Pannexins (Panx) are a family of proteins that share sequences with the invertebrate gap junction proteins, innexins, and have a similar structure to that of the vertebrate gap junction proteins, connexins. To date, the Panx family consists of 3 members, but their genetic sequences have only been completely determined in a few vertebrate species. Moreover, expression of the Panx family has been...
متن کاملMolecular cloning and characterization of chitinase genes from Candida albicans.
Chitinase (EC 3.2.1.14) is an important enzyme for the remodeling of chitin in the cell wall of fungi. We have cloned three chitinase genes (CHT1, CHT2, and CHT3) from the dimorphic human pathogen Candida albicans. CHT2 and CHT3 have been sequenced in full and their primary structures have been analyzed: CHT2 encodes a protein of 583 aa with a predicted size of 60.8 kDa; CHT3 encodes a protein ...
متن کاملSeed Transcriptomics Analysis in Camellia oleifera Uncovers Genes Associated with Oil Content and Fatty Acid Composition
Camellia oleifera is a major tree species for producing edible oil. Its seed oil is well known for the high level of oleic acids; however, little is known regarding the molecular mechanism of lipid biosynthesis in C. oleifera. Here, we measured the oil contents and fatty acid (FA) compositions at four developmental stages and investigated the global gene expression profiles through transcriptom...
متن کاملMolecular Cloning, Characterization, and Expression of Cuc m 2, a Major Allergen in Cucumis melo
Background: Several studies reported the clinical features of IgE-mediated hypersensitivity after ingestion of melon. Melon allergy is a common IgE-mediated fruit allergy in Iran. This prompted us to investigate immunochemical and molecular properties of the major allergen in melon fruit, to compare the IgE-binding capacity of the natural protein with the recombinant allergen, and to determine ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Brazilian Journal of Botany
سال: 2021
ISSN: ['0100-8404', '1806-9959']
DOI: https://doi.org/10.1007/s40415-020-00691-8